Invastigation of 16S rRNA, mecA and nuc genes in coagulase-positive and negative Staphylococci by Real-Time PCR

Staphylococcus aureus is a Gram-positive, round-shaped bacterium that member of the Firmicutes. It often positive for catalase and nitrate reduction. Pathogenic strains promote infections by producing virulence factors such as potent protein toxins, expression cell-surface binds inactivates antibodies. Sepsis-related to methicillin-resistant S. (MRSA) has significant morbidity high mortality rates (15-30%). The methicillin resistance coded with mecA gene, while sensitivity nuc they are chromosomal. Similarly, it coagulase gene (Coa). In this study, 16S rRNA identification Real-Time PCR was investigated in forty isolates, which were cultured at different times terms MIC SIR tests. isolates used study determined level their differences (MecA), susceptibility (Nuc), (Coa) intra-species examined. As result spp. yielded results gene-specific primers all isolates. analysis SYBRGreen-based performed primers, samples confirmed be Staphylococcus. Analysis family followed Coa, Nuc, MecA results, found that, Coa Nuc genes, 19 21 negative. 16 according sigmoidal curves single peak melting values, whereas 24 thought will benefit community contributing rapid effective diagnosis treatment caused negative Staphylococci.